TIDE is a simple and accurate assay to precisely determine the spectrum and frequency of targeted mutations generated in a pool of cells by genome editing tools such as CRISPR/Cas9, TALENs and ZFNs. TIDE requires only standard molecular biology reagents and involves three simple steps: 1. One pair of standard PCR reactions. 2. One pair of standard capillary ("Sanger") sequencing reactions. 3. Analysis of the two resulting raw sequencing files using a dedicated web tool. The algorithm accurately reconstructs the spectrum of indels from the sequence traces. The web tool reports the precise identity of the mutations and their frequencies in a few intuitive graphs and tables.
View TIDE publication
Although the TIDE web tool does not store or analyze any uploaded data, you may prefer to keep your confidential sequence files local.
Local installation of TIDE on your own servers / private cloud overcomes access problems related to firewall issues.
Ready to use TIDE software package also comes with one year technical support*.
Request TIDE software package via this web app by choosing an option that fits your organization.
Next, the Tech Transfer Office of NKI-AVL will get in touch with you to finalize the license agreement.
Done! Your account will be created and you can download the ready to deploy TIDE package.
Academic/Research Institutes & Universities 1 year of tech support* 2500**
Small/Medium Enterprises with < 100 people 1 year of tech support* 5000**
Large Corporations with > 100 people 1 year of tech support* 10000**
*Continued tech support & free updates of new TIDE versions after the initial one year term can be secured against an annual fee that is 15% of the initial license fee.
**The quoted prices include an optional 20% discount that is applied in exchange for permission to use your organization's name/logo solely in connection with the fact that you entered into a license agreement for TIDE with us.
Please provide your contact information below. Our Technology Transfer Office will contact you as soon as possible.
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Required platform: Web Server Required Software:
Required platform: Any local machine from where you can deploy to the shinyapps.oi cloud. Required Software: