METHOD FOR EASY QUANTITATIVE ASSESSMENT OF GENOME EDITING

Free webtool
Local installation of TIDE

TIDE published at Nucleic Acid Research
TIDE developed at Netherlands Cancer Institute - NKI

TIDE: Tracking of Indels by Decomposition


TIDE is a simple and accurate assay to precisely determine the spectrum and frequency of targeted mutations generated in a pool of cells by genome editing tools such as CRISPR/Cas9, TALENs and ZFNs. TIDE requires only standard molecular biology reagents and involves three simple steps: 1. One pair of standard PCR reactions. 2. One pair of standard capillary ("Sanger") sequencing reactions. 3. Analysis of the two resulting raw sequencing files using a dedicated web tool. The algorithm accurately reconstructs the spectrum of indels from the sequence traces. The web tool reports the precise identity of the mutations and their frequencies in a few intuitive graphs and tables.

View TIDE publication

Getting Started with acquiring TIDE Software Package


Benefits of TIDE local installation

Data Security

Although the TIDE web tool does not store or analyze any uploaded data, you may prefer to keep your confidential sequence files local.

No Firewall/Connection Issues

Local installation of TIDE on your own servers / private cloud overcomes access problems related to firewall issues.

Technical Support

Ready to use TIDE software package also comes with one year technical support*.

How does this work?

Step 1 > Request TIDE

Request TIDE software package via this web app by choosing an option that fits your organization.

Step 2 > License Agreement

Next, the Tech Transfer Office of NKI-AVL will get in touch with you to finalize the license agreement.

Step 3 > Download TIDE

Done! Your account will be created and you can download the ready to deploy TIDE package.

What are my options?

Academic

Academic/Research Institutes & Universities
1 year of tech support*
2500**

Small Medium Enterprises

Small/Medium Enterprises with < 100 people
1 year of tech support*
5000**

Corporate

Large Corporations with > 100 people
1 year of tech support*
10000**

If you have any question

Contact Us

*Continued tech support & free updates of new TIDE versions after the initial one year term can be secured against an annual fee that is 15% of the initial license fee.

**The quoted prices include an optional 20% discount that is applied in exchange for permission to use your organization's name/logo solely in connection with the fact that you entered into a license agreement for TIDE with us.

Request TIDE Software Package

Please provide your contact information below. Our Technology Transfer Office will contact you as soon as possible.


**all fields are mandatory








Technical Requirements for TIDE Local Installation


Deploy TIDE on Shiny R Server

Required platform: Web Server
Required Software:

  • Web Server OS: Ubuntu 12.04 or later / Fedora 19+ / RedHat 7+ / openSUSE 13.1+ (For installing the Shiny Server binary 64 bit architecture is needed)
  • R version: 3.1.1 or later
Comments:
  • Installing Shiny Server on Amazon linux instances (e.g. EC2 t2 micro) is also possible. But make sure you have a compatible linux OS (e.g. ‘Amazon Linux AMI’).
  • The required R packages from TIDE and shiny package works with R version 3.0.2 or higher but it is highly recommended to get a version of R 3.1.1 for a smoother deployment of TIDE shiny R package.

Deploy TIDE on shinyapps.io Cloud Server

Required platform: Any local machine from where you can deploy to the shinyapps.oi cloud.
Required Software:

  • Local Machine OS: Linux/Windows/Mac OSX
  • R version: 3.1.1 or later
Comments:
  • You would require an account in shinyapps.io cloud server and you can choose from plans (Free, Basic, Standard or Professional) for your need.

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